Laboratory Support Services

Capralogics Inc. offers a variety of laboratory support services for:

ELISA

Enzyme-Linked ImmunoSorbant Assay (ELISA) is a procedure that is used to detect the presence of an antibody or an antigen in a sample. It can be used to measure or track the progress of your antibody project to see whether an antibody response against the antigen has occurred or is increasing or decreasing over time.  Test serum is run alongside with pre-immune or baseline serum.

Please contact us for additional information.

Antibody Purification

Capralogics Inc. offers several methods of purification for you to choose from as a finish step to your antibody project. We would be happy to work with you to determine the best approach for your specific needs. Please do not hesitate to contact us if you have any questions regarding affinity purification.  Inquire for bulk purification prices.

Peptide Affinity Purification: 

Lyophilized peptide in the range of 15 to 25 mg is preferred for column construction for the purification of 50 ml antisera. The yield of peptide specific antibody is based on quality of immune response and can range from 5 to 10 % of total immunoglobulin. Generally, a minimum of 10 mg of antibody can be obtained.

Protein Affinity Purification: 

About 6 to 10 mg of protein is needed to couple to the affinity matrix column for purification of 30 ml antisera. The protein should not contain Tris or glycine buffers. The yield of protein specific antibody is based on quality of immune response and can range from 5 to 10 % of total immunoglobulin. Generally, a minimum of 10 mg of antibody can be obtained.

Protein A/G Purification: 

Purification of up to 30 ml antiserum by Protein A/G Affinity Chromatography. Most serum yields between 10 to 20 IgG per ml.

IgY Purification: 

Precipitation and purification of total IgY from 10 yolks.

IgY Affinity Purification: 

Labeling of Antibodies: Inquire for pricing

We offer labeling to the following agents: Alkaline Phosphatase, Biotin, and Horseradish Peroxidase (HRP). Please inquire for pricing or additional options of labeling your purified antibodies.