Let our team assist you from the start to ensure the success of your custom antibody project! There must be many things taken into consideration before starting an antibody program in any species. With over 30 years of experience let one of staff members assist you in developing a plan that is tailored to meet your specific needs.
Antigen Design
Let our highly skilled staff assist you with your antigenic selection and design. When producing antibodies it is important to take into consideration a number of factors:
- What application(s) do you intend to use the antibody for
- Choosing the best sequence in relation to your target antigen
- Avoiding epitopes that may be unavailable due to structural constraints
- Accessing any possible wanted or un-wanted cross reactivity
Antigenic peptide epitopes can be selected using software available at The Immune Epitope Database and Analysis Resource (IEDB) and also lists of ranked peptide epitopes can subsequently searched with BLAST. For more information on these services or to submit an antigen of interest for analysis please contact us at info@capralogics.com or 413.477.6866.
Peptide Synthesis
Capralogics Inc. can assist you in synthesizing your peptide of interest for antibody production. For most antibody production projects, a peptide of at least 85% purity is recommended. If requested, we can provide a higher purity antigen for an additional charge. Capralogics will synthesize approximately 15-20mg of peptide, which is generally enough peptide to conjugate for the immunogen, perform ELISA, and construct a peptide affinity matrix column, if desired.
Peptide synthesis will be approximately 20 mg of peptide for peptides up to 25 amino acids in length at >85% purity and will include documentation and HPLC analysis. For peptides larger than 25 amino acids there will be an additional fee for synthesis.
Peptide acetylation and amidation are offered for an additional fee. Other modifications are available upon request and all synthesis will be quoted on an individual basis. Get started today, e-mail your amino acid sequence to info@capralogics.com or fill out our Service Order Form.
Conjugation
To increase the immunogenicity of your antigen you may need to couple it to a larger carrier protein. Generally peptides less than 13-15 KD or less than 25-27 amino acids may need to be conjugated. You can attach your antigen to KLH, BSA or other carrier proteins upon request. Peptides may need to have a terminal end Cysteine (C) added to the peptide sequence in order to perform conjugation.
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