capralogics
Sheep anti-5-Methyl Cytosine (5-mC)

DATASHEET

Description: An antibody to 5-methyl cytosine antibody is valuable for studying DNA methylation.  Publications and product literature over the years abbreviate 5-methyl cytosine as 5mC, m5C, 5-Me, and 5-MeC.

Specifications: This antibody product was tested using immunoprecipitation against 5-mC and bromodeoxyuridine (BrdU) along with no antigen control and assayed by A 405 nm spectrophotometry as shown in the graph below.

Formulation: 250 µg of purified IgG in PBS with no preservatives, antisera to 5-methyl cytosine is available for sale

Storage: Freeze at -20° C. For long term storage aliquot and freeze at -70° C. Avoid freeze-thaw cycles.

capralogics

Positive staining of rat neuron cells performed with 2% BSA/0.1% Tween 20 in PBS blocking and incubated at 4°C overnight with antibody dilution of 1:200

For Research Purposes Only
info@capralogics.com

FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number P00704
Amount 250 µg
Price buy online
Host Sheep
Antigen

5-methyl Cytosine coupled to coupled to keyhole limpet hemocyanin (KLH)
Purification Sheep polyclonal antibody is provided as a protein G purified immunoglobulin
Concentration 1.9 mg/mL determined by extinction coefficient: O.D. 1.4 at 280 nm equals 1.0 mg/mL
Applications  
Immunoprecipitation 25 - 100 µg/mL
Immunohistochemistry Immunocytochemistry
Immunofluorescence

Frozen and Paraffin sections, starting dilution ~ 10 µg/mL. Use a fixation step in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining with the simultaneous detection of nuclear cytoplasmic and membrane components as well as preservation of morphological detail.

Western and Dot Blot 1:1000

5MeC

Citations and Applications:

Blotting: Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI.   Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology. 2007 Apr;132(4):1476-94  Abstract

Blotting: Katoh M, Curk T, Xu Q, Zupan B, Kuspa A, Shaulsky G.  Developmentally regulated DNA methylation in Dictyostelium discoideum. Eukaryot Cell. 2006 Jan;5(1):18-25  Full Text

Blotting: Park IY, Sohn BH, Choo JH, Joe CO, Seong JK, Lee YI, Chung JH.  Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development. J Cell Biochem. 2005 Feb 15;94(3):585-96  Abstract

Affinity Chromatography and Blotting: Fisher O, Siman-Tov R, Ankri S.  Characterization of cytosine methylated regions and 5-cytosine DNA methyltransferase (Ehmeth) in the protozoan parasite Entamoeba histolytica.  Nucleic Acids Res. 2004 Jan 9;32(1):287-97 Full Text

Immunoprecipitation:  Luo S, Preuss D.  Strand-biased DNA methylation associated with centromeric regions in Arabidopsis.  Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):11133-8  Full Text

Immunocytochemistry: Poehland R, Al-Rostum F, Becker F, Viergutz T, Brunner RM, Kanitz W, Bhojwani S.  Donor Cell Lines Considerably Affect the Outcome of Somatic Nuclear Transfer in the Case of Bovines. J Reprod Dev. 2007 Aug;53(4):737-48 Full Text

Immunocytochemistry: Mohana Kumar B, Jin HF, Kim JG, Song HJ, Hong Y, Balasubramanian S, Choe SY, Rho GJ.  DNA methylation levels in porcine fetal fibroblasts induced by an inhibitor of methylation, 5-azacytidine. Cell Tissue Res. 2006 Sep;325(3):445-54  Abstract

Immunohistochemistry: Loukinov DI, et al.  BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma.  Proc Natl Acad Sci U S A. 2002 May 14;99(10):6806-11  Full Text