anti-Adiponectin Receptor AdipoR1 C-terminus
$345.00
Amount: 200 ug
Synonyms: Adiponectin Receptor, ADPR1, AdipoR1
Specifications
Host Species: Goat
Antigen: 25 amino acid (aa) synthetic peptide VAAAFVHFYGVSNLQEFRYGLEGGC corresponding to aa 345-369 of the C-terminal domain of AdipoR1
Reactive Species: Highly conserved suggesting multiple species
Format/Conjugates/Purification: Peptide Affinity Purification
Buffer & Formulation: Affinity purified antibody @ 1mg/ml in 10 mM KHPO4, 140 mM NaCl, BSA @ 1mg/ml and 0.1 % sodium azide
Storage: Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone: Polyclonal
Applications
Western Blotting: 1:1000
IHC: 1:250 (paraffin sections)
References
Kadowaki T, Yamauchi T, Kubota N, Hara K, Ueki K, Tobe K. Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome. J Clin Invest. 2006 Jul;116(7):1784-92. Full Text
Rasmussen MS, Lihn AS, Pedersen SB, Bruun JM, Rasmussen M, Richelsen B. Adiponectin receptors in human adipose tissue: effects of obesity, weight loss, and fat depots. Obesity (Silver Spring). 2006 Jan;14(1):28-35. Full Text
Staiger H, Kaltenbach S, Staiger K, Stefan N, Fritsche A, Guirguis A, Peterfi C, Weisser M, Machicao F, Stumvoll M, Haring HU. Expression of adiponectin receptor mRNA in human skeletal muscle cells is related to in vivo parameters of glucose and lipid metabolism. Diabetes. 2004 Sep;53(9):2195-201. Full Text
Yamauchi T, et al. Cloning of adiponectin receptors that mediate antidiabetic metabolic effects. Nature. 2003 Jun 12;423(6941):762-9. Abstract
Comments
Peptide sequence is < 50 % identical to other receptors in this region. The antibody recognizes AdipoR1 in multiple species since the sequence region recognized by the antibody is highly conserved.
Sequence Alignment between human, mouse and rat:
345VAAAFVHFYGVSNLQEFRYGLEGGC369Human
345VAAAFVHFYGVSNLQEFRYGLEGGC369Mouse
345VAAAFVHFYGVSNLQEFRYGLEGGC369Rat