Specifications
Host Species: Sheep
Antigen: Immunogen was brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA prepared by David Stollar's laboratory at Tufts University. The sheep was immunized with sera collections, and IgG purifications at Capralogics, Inc. in Hardwick, Massachusetts. This polyclonal antibody is available from resellers and from Capralogics directly.
Reactive Species: Highly conserved suggesting multiple species
Format/Conjugates/Purification: Protein G
Buffer & Formulation: Purified IgG in PBS with no preservatives
Storage: Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone: Polyclonal
Applications
IHC: 1:200 immunofluorescent staining
Immunoprecipitation: 8 ug/ml
References
Lei, Yuanjiu, et al. “Cooperative sensing of mitochondrial DNA by ZBP1 and cGAS promotes cardiotoxicity.” Cell (2023) Full Text IF
Zhang T, Yin C, Boyd DF, Quarato G, Ingram JP, Shubina M, Ragan KB, Ishizuka T, Crawford JC, Tummers B, Rodriguez DA. Influenza virus Z-RNAs induce ZBP1-mediated necroptosis. Cell. 2020 Mar 19;180(6):1115-29. Full Text IP, IF
Leonova K, Safina A, Nesher E, Sandlesh P, Pratt R, Burkhart C, Lipchick B, Gitlin I, Frangou C, Koman I, Wang J. TRAIN (Transcription of Repeats Activates INterferon) in response to chromatin destabilization induced by small molecules in mammalian cells. Elife. 2018 Feb 5;7:e30842. Full Text IF
Cerna A, Cuadrado A, Jouve N, Diaz de la Espina SM, De la Torre C. Z-DNA, a new in situ marker for transcription. Eur J Histochem. 2004;48(1):49-56. Abstract IF
Herbert A, Rich A., Left-handed Z-DNA: structure and function. Genetica. 1999;106(1-2):37-47. Abstract IP
