Specifications
Host Species: Rabbit
Antigen: Synthetic peptide CVLETRSPNPSILSLTWQDEYEELQDQETF corresponding to 35-65 residues of N-terminus of mouse IL-21 receptor
Reactive Species: Mouse, Human not tested
Format/Conjugates/Purification: Affinity Purified
Buffer & Formulation: Affinity purified antibody in 10 mM KHPO4, 140 mM NaCl, BSA @ 1mg/ml and 0.1 % sodium azide
Storage: Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone: Polyclonal
Applications
ELISA: 1:50,000
IHC: 1:250
Flow Cytometry: 1:25
References
Comes A, Rosso O, Orengo AM, Di Carlo E, Sorrentino C, Meazza R, Piazza T, Valzasina B, Nanni P, Colombo MP, Ferrini S. CD25+ regulatory T cell depletion augments immunotherapy of micrometastases by an IL-21-secreting cellular vaccine. J Immunol. 2006 Feb 1;176(3):1750-8. Abstract
Comments
This F(ab’)2 antibody recognizes mouse interleukin-21 receptor and the peptide sequence is less than 50% similiar to other sequences in GenBank in this region. CI0156 was not tested for cross-reactivity to human IL-21 receptor.
Sequence Alignment of between mouse and human IL-21 receptor:
CVLETRSPNPSILSLTWQDEYEELQDQETF Mouse IL-21 receptor CILEMWNLHPSTLTLTWQDQYEELKDEATS Human IL-21 Receptor