CI0172: Immunohistochemistry with Mouse CCR9 biotin conjugated antibody on paraffin section of mouse spleen.

CCR9 (Mouse) Biotin conjugated IgG

Name: CCR9 (Mouse) Biotin conjugated IgG
SKU: CI0172
Availability: InStock

$345.00

SKU: CI0172 Category: Chemokine Receptor

Amount: 100 ug

Synonyms: C-C chemokine receptor type 9 (C-C CKR-9), CC-CKR-9, CCR-9, GPR-9-6, G-protein coupled receptor 28, and CDw199 antigen

Specifications

Host Species: Goat

Antigen: Synthetic peptide IPGMFDDFSYDSTASTDDYMNLNFSSFF corresponding to aa 10-37 of mouse CCR9

Reactive Species: Human, Mouse

Format/Conjugates/Purification: Peptide Affinity Purification

Buffer & Formulation: 10 mM KHPO4, 140 mM NaCl, BSA @ 1mg/ml and 0.1 % sodium azide

Storage: Aliquot and freeze at -80° C. Stable for 3 years when stored at -80C. Avoid freeze-thaw cycles.

Clone: Polyclonal

Applications

ELISA: 1:100000

Western Blotting: 2ug/ml

IHC: 8ug/ml

Comments

Peptide sequence is <50% identical to other mouse chemokine receptors in this region. This antibody recognizes mouse CCR9 and cross reacts to human CCR9 with approximately 50% less titer.

Quality Control: Immunohistochemistry on mouse spleen

Unconjugated version of this antibody is approved for flow cytometry

References

Marski M, Ye AL, Abraham C. CD18 is required for intestinal T cell responses at multiple immune checkpoints. J Immunol. 2007 Feb 15;178(4):2104-12. Abstract FC
Liu JX, Cao X, Tang YC, Liu Y, Tang FR. CCR7, CCR8, CCR9 and CCR10 in the mouse hippocampal CA1 area and the dentate gyrus during and after pilocarpine-induced status epilepticus. Neurochem 2007 Feb;100(4):1072-88. Abstract IC
Zaballos, A.,. et. al. Identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK. J. Immunol. 162 (10), 5671-5675 (1999)