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anti-human CCR6

$345.00

SKU: CI0129 Category:

Amount: 200 ug

Synonyms: C-C CKR-6, CC-CKR-6, CCR-6, LARC receptor, GPR-CY4, GPRCY4, Chemokine receptor-like 3 (CKR-L3), DRY6, G-protein coupled receptor 29, and CD196 antigen

Specifications

Host Species: Goat

Antigen: Synthetic peptide YFVSVNTSYYSVDSEMLLCSLQEVRQFSR to amino acid residues 18 - 46 of human CCR6

Reactive Species: Human

Format/Conjugates/Purification: Affinity Purified

Buffer & Formulation: Affinity purified antibody in 10 mM KHPO4, 140 mM NaCl, BSA @ 1mg/ml with 0.1 % sodium azide

Storage: Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.

Clone: Polyclonal

Applications

ELISA: 1:37,500

Western Blotting: 1:250

IHC: 1:125

Flow Cytometry: 2-6ul/10^6cells/50-100ul

Comments

Peptide sequence is <50% identical to other human chemokine receptors in this region. The antiserum recognizes human CCR6. Cross reactivity to mouse CCR6. Human CCR6 cross reacts with mouse CCR6 when used in immunohistochemistry, western blotting and other applications where linear epitopes of the corresponding mouse CCR6 are accessible.

References

Caballero-Campo P, Buffone MG, Benencia F, Conejo-García JR, Rinaudo PF, Gerton GL. A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis. J Cell Physiol. 2014 Jan;229(1):68-78. Full Text WB mouse tissue

Veerayutthwilai O, Byers MR, Pham T-TT, Darveau RP, Dale BA. Differential regulation of immune responses by odontoblasts. Oral Microbiol Immunol 2007: 22: 5–13  Abstract IH
Rubie C, Frick VO, Wagner M, Weber C, Kruse B, Kempf K, Konig J, Rau B, Schilling M.Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases.  World J Gastroenterol 2006 12(41):6627-6633 Full Text WB
Rubie C, Frick VO, Wagner M, Rau B, Weber C, Kruse B, Kempf K, Tilton B, Konig J, Schilling M.  Enhanced expression and clinical significance of CC-chemokine MIP-3 alpha in hepatocellular carcinoma.  Scand J Immunol. 2006 Jun;63(6):468-77.  Abstract IH, WB
Rubie C, Oliveira V, Kempf K, Wagner M, Tilton B, Rau B, Kruse B, Konig J, Schilling M.  Involvement of chemokine receptor CCR6 in colorectal cancer metastasis.  Tumour Biol. 2006 27(3):166-74. Abstract  IH, WB

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