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A:  Purified Llama anti-mouse IgG was analyzed on 10% SDS-PAGE under non-reduced (I) and reduced conditions(II). B:   The mouse IgG was transferred to nitrocellulose and blotted with purified llama anti-mouse IgGs. The blot shows that all the fractionated antibody contains three llama subclasses that are reactive to the heavy and light chains of the whole mouse IgG molecule under reduced conditions.
A:  Purified Llama anti-mouse IgG was analyzed on 10% SDS-PAGE under non-reduced (I) and reduced conditions(II). B:   The mouse IgG was transferred to nitrocellulose and blotted with purified llama anti-mouse IgGs. The blot shows that all the fractionated antibody contains three llama subclasses that are reactive to the heavy and light chains of the whole mouse IgG molecule under reduced conditions.

Llama anti-Mouse IgG

$350.00

SKU: PL0121 Category:

Amount: 250ug

Specifications

Host Species: Llama

Antigen: ChromPure Mouse IgG, whole molecule

Format/Conjugates/Purification: Protein A affinity purification

Buffer & Formulation: 5% BSA, 0.1% sodium azide

Storage: Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.

Clone: Polyclonal

Comments

A: Panel I Non-Reduced, Panel II Reduced

Lane #        4 ug Protein loaded                                             

1                Llama IgG1, G2, G3 anti-mouse IgG         
2                Llama IgG1 Fraction anti-mouse IgG                        
3                Llama IgG2 Fraction anti-mouse IgG                      
4                Llama IgG3 Fraction anti-mouse IgG                       

B: ​Western Blot of Llama Anti-Mouse IgG with Mouse IgG

Lane #  Sample(250ng)               Antibody 

1        NR Mouse IgG     Llama IgG1, G2, G3 anti-mouse IgG
2        R- Mouse IgG       Llama IgG1, G2, G3 anti-mouse IgG
3        R- Mouse IgG       Llama IgG1 Fraction anti-mouse IgG
4        R-Mouse IgG        Llama IgG2 Fraction anti-mouse IgG
5        R-Mouse IgG        Llama IgG3 Fraction anti-mouse IgG 

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