Specifications
Host Species: Rabbit
Antigen: Synthetic peptide CHRLPGSYDSTSSDSLYPRGI corresponding to amino acids 29-49 at N-terminus of human pp36, a tyrosine-phosporylated adaptor protein.
Reactive Species: Mouse
Format/Conjugates/Purification: Affinity Purified
Buffer & Formulation: Affinity purified antibody in 10 mM KHPO4, 140 mM NaCl, BSA @ 1mg/ml and 0.1 % sodium azide
Storage: Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone: Polyclonal
Applications
ELISA: 1:50,000
Western Blotting: 1:500
IHC: 1:150
References
Lindquist JA, Simeoni L, Schraven B. Transmembrane adapters: attractants for cytoplasmic effectors. Immunol Rev. 2003 Feb;191:165- 82. Abstract
Weber JR et al 1998 Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T Cells and natural killer cells. J Exp Med 1998, 187, 1157-61. Full Text
Comments
Western Blot: This antibody binds to a ~40 kD protein in Western blotting on mouse lung or human T cell line Jurkat and K562 cells